By Icon Health Publications
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Additional info for Morphine - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References
Therefore, our specific aims for the proposed funding period are to: 1) determine the second messenger(s) mediating the dynorphin and nociceptin effects on Im in CA1 hippocampal neurons. 2) Examine the effects of opioid on pharmacologically-isolated EPSCs and responses to NMDA, AMPA and kainate in CA1 of hippocampus. 3) Perform a battery of pharmacological tests to determine the site of action of chronic morphine treatment and possible changes in the postsynaptic NMDA receptor subunit composition in rat nucleus accumbens neurons.
In addition, our studies in slices of nucleus accumbens core confirmed the opiate reduction of synaptic transmission in the nucleus accumbens and found pronounced interaction of opiates with glutamate receptor agonists, and especially with those acting on non-NMDA and NMDA receptor-mediated neurotransmission, in neurons from rats treated chronically with morphine. Therefore, our specific aims for the proposed funding period are to: 1) determine the second messenger(s) mediating the dynorphin and nociceptin effects on Im in CA1 hippocampal neurons.
The specific aims are designed to test this hypothesis by: 1) determining the brain regions expressing CRE-responsive genes following morphine exposure; 2) monitoring the levels of the prohormone processing enzymes, PC1 and PC2, as well as bioactive peptide hormones in these regions; and 3) determining novel or uncharacterized genes/proteins regulated by various paradigms of morphine exposure in CREresponsive mouse brain regions. The focus of this proposal will be to use two new technologies, ProteinChip Array (SELDI-TOF Mass Spectrophotometry) and Gene Chip Array, to characterize and identify genes and proteins involved in opiate use.
Morphine - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References by Icon Health Publications