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Extra resources for Metabolites - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References
Second, we will explore the effects of IR on synthesis of bioactive metabolites of arachidonic acid. Third, we will examine the relationship between endothelial function and levels of CYP and NO synthase protein. Fourth, we will determine the effect of application of exogenous arachidonic acid metabolites (epoxyeicosatrienoic acids) on vascular tone. These data will determine mechanisms of endothelial dysfunction in IR. Moreover, the specific role of EDHF and mechanisms for its dysfunction will be determined.
Cell-based reporter assays with PPARalpha expression constructs and fatty acyl-CoA oxidase reporter constructs will test their role is ligand/activators for PPARalpha. 2. Characterize the properties of DHEA or any active metabolites involving PPARalpha or the non-classical glucorticoid induction mechanism described by Guzelian and Kasper. Our preliminary data suggests that CYP2Bl and CYP3A are induced by DHEA in vivo, but not by other peroxisome proliferators. Since the CYP4 enzyme subfamily are not good catalysts of PAH metabolism, CYP2Bl and 3A23 must account for enhanced metabolism possibly due to the non-classical mechanism of induction.
For microbial-produced natural products, this is largely the result of the extremely high (99%) rediscovery rate, which occurs as a result of isolation, and testing of the same organisms again and again. This high rediscovery rate supports recent work suggesting that less than 1% of microbial diversity has been cultured and studied experimentally, and that the remaining 99% is not readily amenable to laboratory cultivation. Using this generally accepted theme as a starting point, novel media, media supplements, and cultivation conditions will be utilized to isolate microorganisms from a variety of natural, under-explored environments in Alabama.
Metabolites - A Medical Dictionary, Bibliography, and Annotated Research Guide to Internet References by Icon Health Publications